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Submited on: 13 Sep 2010 11:20:23 PM GMT
Published on: 14 Sep 2010 12:56:31 AM GMT
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Other Comments:
A good overall presentation. This technique will certainly be of great use to other researchers. The style of presentation and the experimental techniques used are of acceptable quality. The author must put in details concerning approval from Animal Ethics committee since work was carried out on animals. The number of animal used should also be clearly mentioned.
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Competing interests:
none
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Invited by the author to review this article? :
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Have you previously published on this or a similar topic?:
No
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References:
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Experience and credentials in the specific area of science:
I work with various cell culture techniques and animal models in cancer research
- How to cite: Abdul Majid A .An efficient approach to enrich glioma stem cells from glioma cell lines in culture [Review of the article 'An efficient approach to Enrich Glioma Stem Cells from Glioma Cell Lines in Culture ' by Kamnasaran D].WebmedCentral 2010;1(12):WMCRW00224
Identifying the role cancer stem cells (CSC) is important in understanding the resistance to standard therapy of surgery, radiation and TMZ. Standard glioma cell lines, U87 has been used for a long time and understanding the role of CSC in these lines is important. The above procedure to isolate CSC will be critical in understanding GBM biology
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Working in glioma stem cells and potential therapeutic applications