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The authors described "High Resolution Melting (HRM)" methods for SNP discovery, which is rapid and cost effective. The authors described a rapid and cost effective methods for SNP discovery, which is "High Resolution Melting (HRM)" Analysis. Short history, principle and comparison among other similar methods were shown.
No, this is one of traditional chemical methods for SNP discovery. Like as the authors described, some commercial products are available.
Yes, but their claims are weak because of their poor originality.
They did not show any experimental results. If they could show some examples of clinical implementation and their usability, their claims would sound powerful.
Valid. The detailed principle of HRM was shown but its methodology is not enough detail to reproduce.
Yes. If clinical applications of HRM were shown, this manuscripts would be better.
No, it is simple summary.
About SNPs discovery method, next generation sequencing (NGS) technologies are very powerful and precise. HRM and other chemical methods are out-of-date. So they should clarify why they introduce this methods right now. I understand HRM is very simple and cheap, and there are some advantages of HRM analysis rather than NGS. The author should describe that by showing their experiences.
Yes, I performed SNPs discovery with Sanger capillary sequencers and Next Generation Sequencers.
I appreciate that the principles and characteristics of High Resolution Melting (HRM) analysis are very well summarized by authors.
No, this paper introduces current HRM analytical methods.
a. I feel like there are repetitive explanations about HR-1 and HRM, in the paragraph of “Innovations from instrument manufactures…..”. Please let us know about application of HRM analysis in SNP typing, clinical study and so on by citing concrete examples.
b. Instead of EvaGreen, LDGreen can be found in the ref. 5.
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