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What are the main claims of the paper and how important are they?
The crop Vigna radiata is an important crop in India. It is indeed a comendable effort to study the genomics of this plant which would go a long way in improving the agronomic values.
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Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.
Yes, these claims are novel.
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Are the claims properly placed in the context of the previous literature?
Not just properly, but very well placed
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Do the results support the claims? If not, what other evidence is required?
The results support the claims.
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If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?
The protocols are quite routine and no major deviations are seen.
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Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?
The methodology is sufficient and the analyses can be reproduced.
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Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?
No
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Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?
Yes it outstanding in the sense that an attempt has been made to create a database of this crop.
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Other Comments:
Nothing in particular.
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Competing interests:
None
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Invited by the author to review this article? :
Yes -
Have you previously published on this or a similar topic?:
No
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References:
None
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Experience and credentials in the specific area of science:
This is an important piece of Bioinformatics subject dealing with database of genomics and I have the necessary expertise in this field.
- How to cite: Ghosh J .Identification and Characterization of EST-SSRs in Mungbean[Review of the article 'Identification and Characterization of EST-SSRs in Mungbean ' by Gacche R].WebmedCentral 2014;5(3):WMCRW003021
Mungbean is orphan crop and as authors highlighted very limited genomic resources are available. In present study Authors have evaluated microsatellites identified in ESTs. Since, the number of ESTs and GSS are very limited so not significant to test for microsatellite evolution or characterisation per se. I suggest Author to try for development of markers based on EST-SSR in Mungbeans.
Claims are novel- no previous significant efforts have been made toward EST-SSR development in Mungbean
Yes
Statistical significance, and comparison with other legumes required
NA
Yes
Author can try characterisation of SSRs in species evolutionary close to mungbean
Not a outstanding, SSR mining is routine practice, It could be interesting if author could review genomic resources for orphan crops like mungbean
Need to be correct typo mistakes
None
Yes
Yes
Singh, H., Deshmukh, R. K., Singh, A., Singh, A. K., Gaikwad, K., Sharma, T. R., ... & Singh, N. K. (2010). Highly variable SSR markers suitable for rice genotyping using agarose gels. Molecular breeding, 25(2), 359-364.
Working on SSR mining from last 10 years