Submited on: 27 Oct 2010 05:35:20 PM GMT
Published on: 27 Oct 2010 09:30:46 PM GMT
 
Hepatic Cytochrome P450 4a Expression Level In A Rat Model Of Microvesicular Steatosis
Posted by Ms. Rui Liu on 02 Nov 2017 02:27:26 AM GMT Reviewed by Interested Peers

  • What are the main claims of the paper and how important are they?

    Microvesicular steatosis induced CYP4A upregulation in liver.

    The results may indicate expression of metabolic enzymes change during hepatic steatosis and this may result in an altered drug metabolism status during liver diseases.


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    Yes, for this subtype CYP4A of CYP450s in this hepatic steatosis model.


  • Are the claims properly placed in the context of the previous literature?

    Not really.


  • Do the results support the claims? If not, what other evidence is required?

    Yes, but not adequate. For example, the researchers did not explain any mechanisms for this phenomenon they have observed. Which pathway is involved and which protein may account for the upregulation of CYP4A.


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    Not applicable for this research article.


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    It’s valid. Yes, there are details regarding the methods.


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    The model is induced by using short-term intake of diets containing Orotic acid and conducted in rats. Typically long-term high fat diet induced mouse model is more reliable and obtain a better simulation for hepatic steatosis in human.


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    No. There are a lot of decent articles with high quality results which published on journal of hepatology in this research area.  The data from this paper is informative but very limited.


  • Other Comments:

    For the research background, the author introduced steatosis and lipid metabolism in liver under diet-induced model including the role of L-FAB and PPAR, however he/she failed to state the reason why they pay attention to CYP450 4A in this specific model, how CYP450 4A expression might be related with aforementioned regulators

    Their technique for presenting research data is obsolete, for showing the weight mass changes in Table 1&2, graph is preferable than just listing the numbers.

    The potential value of this research for finding the raising expression level of CYP4A in liver steatosis model is not well-defined, also there is no further elaboration for this phenomenon or complementary statement for demonstrating the demerits of this study.

  • Competing interests:
    .
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    .

  • How to cite:  Liu R .Hepatic Cytochrome P450 4a Expression Level In A Rat Model Of Microvesicular Steatosis[Review of the article 'Hepatic Cytochrome P450 4a Expression Level In A Rat Model Of Microvesicular Steatosis ' by Abdul Majid A].WebmedCentral 2017;8(11):WMCRW003385
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Untitled
Posted by Prof. Khalid Abu-Salah on 18 Dec 2010 02:40:30 AM GMT

  • Other Comments:

    The work is interesting and worth publishing, nevertheless, the role of P450 4A under norml conditions, is not well explained, neither its relationship to microvesicular steatosis.
    Tables 1 and 2, and Figs. 1 and 2 could not be seen with the article.
    Thank you,
    Khalid M. Abu-Salah

  • Invited by the author to review this article? :
    Yes
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:
    None
  • How to cite:  Abu-Salah K .Untitled[Review of the article 'Hepatic Cytochrome P450 4a Expression Level In A Rat Model Of Microvesicular Steatosis ' by Abdul Majid A].WebmedCentral 2017;1(12):WMCRW00257
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1 Is the subject of the article within the scope of the subject category? Yes
2 Are the interpretations / conclusions sound and justified by the data? Yes
3 Is this a new and original contribution? Yes
4 Does this paper exemplify an awareness of other research on the topic? Yes
5 Are structure and length satisfactory? Yes
6 Can you suggest brief additions or amendments or an introductory statement that will increase the value of this paper for an international audience? Yes
7 Can you suggest any reductions in the paper, or deletions of parts? Yes
8 Is the quality of the diction satisfactory? Yes
9 Are the illustrations and tables necessary and acceptable? Yes
10 Are the references adequate and are they all necessary? Yes
11 Are the keywords and abstract or summary informative? Yes
  • Other Comments:

    the auther may need to clarify the aim of the work in the introduction part more clearly ad also to focus more on the sigifact of the findings in the result and descussion part.

  • Competing interests:
    no
  • Invited by the author to review this article? :
    Yes
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    i am teacing toxogolgy to the pharmacy students. I aslo conducted some animal toxogological studies for some anticancer agents.

  • How to cite:  Zihlif M .Hepatic Cytochrome P450 4a Expression Level In A Rat Model Of Microvesicular Steatosis [Review of the article 'Hepatic Cytochrome P450 4a Expression Level In A Rat Model Of Microvesicular Steatosis ' by Abdul Majid A].WebmedCentral 2017;1(12):WMCRW00218
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