Submited on: 17 Dec 2010 10:08:17 AM GMT
Published on: 18 Dec 2010 11:58:58 AM GMT
 

1 Is the subject of the article within the scope of the subject category? Yes
2 Are the interpretations / conclusions sound and justified by the data? Yes
3 Is this a new and original contribution? No
4 Does this paper exemplify an awareness of other research on the topic? Yes
5 Are structure and length satisfactory? Yes
6 Can you suggest brief additions or amendments or an introductory statement that will increase the value of this paper for an international audience? Yes
7 Can you suggest any reductions in the paper, or deletions of parts? No
8 Is the quality of the diction satisfactory? Yes
9 Are the illustrations and tables necessary and acceptable? Yes
10 Are the references adequate and are they all necessary? Yes
11 Are the keywords and abstract or summary informative? Yes
  • Other Comments:

    The review paper by Labinskyy et all addresses a very important issue, namely, the interaction of diabetes mellitus and exercise to regulate vascular function. The mechanisms underlying endothelial dysfunction of diabetes mellitus are likely multifaceted and may include an increased oxidative/nitrosative stress, a relative deficiency of reduced tetrahydrobioptrerin associated with increased eNOS uncoupling, increased activity of arginase competing with eNOS for the common substrate, L-arginine; elevated levels of the endogenous inhibitor ADMA, and quenching of NO by advanced glycosylation products. The authors provide an extensive overview of the relevant literature and highlight several controversies in the current research. It would be interesting to critically discuss the ideas put forward in recent studies that there is a ‘protected’ transport mechanism for NO in the blood, taking NO from areas of high production to be released at critical areas of the circulation (e.g. Stabler T et all, Ann. N.Y. Acad. Sci.1203(2010)101-106). According to this theory, in vivo NO bioavailability may be regulated by formation of NO-containing compounds in the blood and NO may be transported throughout the vasculature in both the plasma and red blood cells. In diabetes increases in advanced glycation end products and reactive oxygen species can significantly modify these transport mechanisms and contribute to vascular dysfunction.

    Although there is clinical and experimental evidence that exercise improves cardiovascular function of diabetes, the exact mechanisms responsible for these beneficial effects are not completely understood. The authors provide a thorough discussion of potential pathways mediating the beneficial effects of exercise focusing of the regulation of endothelial nitric oxide synthase and exercise-induced temporospatial increases in NO bioavailability. It would be also interesting to discuss the effect of arginase activity on vascular function in more detail. For example, recent studies raised the possibility that an enhanced flux of amino acids to vascular endothelial cells may contribute to the beneficial cardiovascular effects of exercise by increasing the intracellular production of NO (e.g. Newsholme P, Clin Sci  (2010) 118, 341-349).

    The discussion highlights a highly significant and often overlooked aspect of diabetes induced vascular dysfunction: the interaction of diabetes and aging. Diabetes affects approximately one in five persons aged 65 years or older, and as the population ages, the impact of diabetes will intensify. Furthermore, there is increasing evidence that the aged vasculature exhibit increased sensitivity to the deleterious effects of metabolic diseases.

    Taken together this is thorough review of the current literature summarizing the regulation of eNOS, its impaired function in diabetic cardiovascular dysfunction and the pathways regulated by exercise counteracting it.

  • Competing interests:
    No.
  • Invited by the author to review this article? :
    Yes
  • Have you previously published on this or a similar topic?:
    Yes
  • References:
    None
  • Experience and credentials in the specific area of science:

    My main focus of research is aging -induced vascular dysfunction and the involvement of oxidative stress and inflammation.

  • How to cite:  Csiszar A .The interplay of diabetes and exercise in vascular dysfunction[Review of the article 'The Contrary Impact Of Diabetes And Exercise On Endothelial Nitric Oxide Synthase Function ' by Edwards J].WebmedCentral 2011;2(2):WMCRW00497
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1 Is the subject of the article within the scope of the subject category? Yes
2 Are the interpretations / conclusions sound and justified by the data? Yes
3 Is this a new and original contribution? Yes
4 Does this paper exemplify an awareness of other research on the topic? Yes
5 Are structure and length satisfactory? Yes
6 Can you suggest brief additions or amendments or an introductory statement that will increase the value of this paper for an international audience? Yes
7 Can you suggest any reductions in the paper, or deletions of parts? No
8 Is the quality of the diction satisfactory? Yes
9 Are the illustrations and tables necessary and acceptable? Yes
10 Are the references adequate and are they all necessary? Yes
11 Are the keywords and abstract or summary informative? Yes
  • Other Comments:

     

    The contrary impact of diabetes and exercise on endothelial nitric oxide synthase function

    Article ID: WMC001376

    Article Type: Review articles

    This review is very well written and highly relevant. It is concise and focused in the main theme of discussion, the relevance and endothelial nitric oxide synthase function and its regulation by diabetes and exercise.

    Comments:

    Page 3, left column line 18-21, the authors should reformulate the sentence in order to clarify this point.

    Page 3, right column, 2nd paragraph, line 9, please change the symbol O3/4 of superoxide anion for the appropriate one.

    Page 3, right column, 2nd paragraph, line 12-14; other authors have shown that “Oxidant stress in the form of superoxide was significantly increased in the Goto-Kakizaki vasculature”[1], the reference should be included as well.

    [1] Sena, C. M.; Nunes, E.; Louro, T.; Proenca, T.; Fernandes, R.; Boarder, M. R.; Seica, R. M. Br J Pharmacol, 153 (5): 894-906 (2008)

    Page 4 line 9-10. Moderate exercise is beneficial while high intensity forms of exercise are problematic for NIDDM patients. This should also be considered and discussed by the authors.

     

    The contrary impact of diabetes and exercise on endothelial nitric oxide synthase function

    Article ID: WMC001376

    Article Type: Review articles

    This review is very well written and highly relevant. It is concise and focused in the main theme of discussion, the relevance and endothelial nitric oxide synthase function and its regulation by diabetes and exercise.

    Comments:

    Page 3, left column line 18-21, the authors should reformulate the sentence in order to clarify this point.

    Page 3, right column, 2nd paragraph, line 9, please change the symbol O3/4 of superoxide anion for the appropriate one.

    Page 3, right column, 2nd paragraph, line 12-14; other authors have shown that “Oxidant stress in the form of superoxide was significantly increased in the Goto-Kakizaki vasculature”[1], the reference should be included as well.

    [1] Sena, C. M.; Nunes, E.; Louro, T.; Proenca, T.; Fernandes, R.; Boarder, M. R.; Seica, R. M. Br J Pharmacol, 153 (5): 894-906 (2008)

    Page 4 line 9-10. Moderate exercise is beneficial while high intensity forms of exercise are problematic for NIDDM patients. This should also be considered and discussed by the authors.

  • Competing interests:
    No competing interest.
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    I work in these area. Diabetes and vascular function.

  • How to cite:  Sena C .The contrary impact of diabetes and exercise on endothelial nitric oxide synthase function[Review of the article 'The Contrary Impact Of Diabetes And Exercise On Endothelial Nitric Oxide Synthase Function ' by Edwards J].WebmedCentral 2011;2(1):WMCRW00333
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Diabetes is widespread health problem
Posted by Prof. Nataliya A Babenko on 19 Dec 2010 09:19:16 AM GMT

1 Is the subject of the article within the scope of the subject category? Yes
2 Are the interpretations / conclusions sound and justified by the data? Yes
3 Is this a new and original contribution? Yes
4 Does this paper exemplify an awareness of other research on the topic? Yes
5 Are structure and length satisfactory? Yes
6 Can you suggest brief additions or amendments or an introductory statement that will increase the value of this paper for an international audience? No
7 Can you suggest any reductions in the paper, or deletions of parts? No
8 Is the quality of the diction satisfactory? Yes
9 Are the illustrations and tables necessary and acceptable? No
10 Are the references adequate and are they all necessary? Yes
11 Are the keywords and abstract or summary informative? Yes
  • Other Comments:

    Diabetes is widespread health problem. Systemic complications related to vascular dysfunction are now recognized. Taking into account that the management of diabetes and vascular dysfunction is unsolved problem the authors pay attention in the paper on the effects of exercise on the diabetic microvasculature and try to clear the understanding of the the molecular mechanisms that are influenced by diabetes and exercise.

  • Competing interests:
    None
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    We investigate the metabolic problems at different age.

  • How to cite:  Babenko N A.Diabetes is widespread health problem[Review of the article 'The Contrary Impact Of Diabetes And Exercise On Endothelial Nitric Oxide Synthase Function ' by Edwards J].WebmedCentral 2011;1(12):WMCRW00263
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