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Reduction of ovariectomy-induced bone loss in rats by menaquinone-4, cholecalciferol, and (n-3) polyunsaturated fats
By Dr. Robert Davidson , Mr. David Hunt
Submited on: 04 Oct 2010 09:06:45 PM GMT
Published on: 05 Oct 2010 08:07:19 PM GMT
 
Exogenous vitamin K with vitamin D or (n-3) PUFA ameliorate the bone mineral loss associated with age. (Rats)
Posted by Dr. Bill Misner on 16 Jul 2012 03:08:49 PM GMT

  • What are the main claims of the paper and how important are they?

    Menopause or ovariectomization lead to a rapid loss of bone mineralization. Menaquinone-4 may act in conjunction with other nutrients, specifically vitamin D3 or (n-3) polyunsaturated fatty acids (PUFA), to affect bone mineral loss in ovariectomized state. Two experiments on 6-month-old ovariectomized rats placed on diets containing normal or supraphysiological levels of menaquinone-4 while varying the dietary vitamin D3 or (n-3) PUFA for eight months. Femur bone mineral density on Rats fed either a high n-3/n-6 ratio diet or supraphysiological levels of menaquinone-4 for eight months  demonstrated that supraphysiological MK4 in conjunction with either VD3 or (n-3) PUFA supplementation decelerates ovariectomy-induced bone loss in rats. This finding has direct implications in humans. Exogenous vitamin K with vitamin D or (n-3) PUFA mayreduce bone mineral loss associated with human female age. Ho-Pham et al., (2012) showed that Vegan diet [rich in vitamin K] did not have adverse effect on bone loss and fracture and that high intakes of animal protein and animal lipids were negatively associated with bone loss. Ho-Pham LT, Vu BQ, Lai TQ, Nguyen ND, Nguyen TV. Vegetarianism, bone loss, fracture and vitamin D: a longitudinal study in Asian vegans and non-vegans. Eur J Clin Nutr. 2012 Jan;66(1):75-82.


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    Some of these findings are novel. PUFA (n-3) and Vitamin K [MK4] together appear to exhibit a detrimental effect on bone strength qualities. Any significant effect of either nutrient alone was negated when combined with the other nutrient. Considering both nutrients reduce bone resorption this was an unanticipated [novel] effect.


  • Are the claims properly placed in the context of the previous literature?

    Yes, the claims are properly supported and listed in context.


  • Do the results support the claims? If not, what other evidence is required?

    The results support the claims though novel claims listed the author states more research is needed to confirm and identify mechanisms.


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    The authored explanation for this protocol is complete requiring no additional detail.


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    The methodology is valid and could easily be reproduced.


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    One effect may be studied; what is the effect of exercise-imposed gravity on an additional group?


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    This study demonstrates that supraphysiological MK4 in conjunction with either VD3 or (n-3) PUFA supplementation decelerates ovariectomy-induced bone loss in the femur of  rats. This research showed that either supraphysiological doses of MK4 or high (n-3) PUFA supplementation preserves the bending strength and stiffness of the femur in ovariectomized rats. The author demonstrated that supraphysiological MK4 in conjunction with either VD3 or (n-3) PUFA supplementation decelerates ovariectomy-induced bone loss in rats. This finding may have direct implications for humans.


  • Other Comments:

    None.

  • Competing interests:
    None.
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
  • Experience and credentials in the specific area of science:

    AAMA Board Certified Alternative Medicine Practitioner-Holistic Nutrition MS/PhD American Alternative Medical Association Commission on Certification

  • How to cite:  Misner B .Exogenous vitamin K with vitamin D or (n-3) PUFA ameliorate the bone mineral loss associated with age. (Rats)[Review of the article 'Reduction of ovariectomy-induced bone loss in rats by menaquinone-4, cholecalciferol, and (n-3) polyunsaturated fats ' by Hunt D].WebmedCentral 2012;3(7):WMCRW002093
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