Brief Biography:
I see myself as an active contributor to a team of dedicated and ambitious people working on projects that involve blend of Molecular Biology, virology, Cancer Biology, Immunology and Biotechnology to enhance my knowledge and personality. I have a strong motivation to pursue career especially in frontier areas of virology, Cancer Biology, Molecular Biology, Immunology and Biotechnology.\2010 PhD Thesis (Molecular Biology)
National Centre of Excellence in Molecular Biology (CEMB),
University of the Punjab, Lahore.
PhD. Research Project:
?Comparative Study to Evaluate the Effect of Structural Genes of HCV Genotype 1a and 3a in HCV Pathogenesis? (http://eprints.hec.gov.pk/6115/)
2006 PhD Molecular Biology (Course work 70.76%)
National Centre of Excellence in Molecular Biology (CEMB),
University of the Punjab, Lahore Pakistan ? Therapeutic Application of siRNA in Allergic Inflammation
? HCV and Host genes interaction involved in HCV induced Steatosis
? HCV Core and E2 effects on cellular genes involved in Apoptosis
? Protein profiling of ER (Estrogens receptors) and PR (progesterone receptors) in tumor and normal tissue of Breast Cancer patients.
? Mutation analysis VEGF and ANGPT2 and finding association between VEGF (Vascular endothelial growth factor) 460 C/T and 936 C/T of and ANGPT2 Angiopoietin2 axon 4 G/A polymorphism in ovarian cancer of Pakistani population.
? Analysis of HPV and its typing by Path vision HPV kit and Identification of HPV by PCR in cervic.al carcinoma.
? Optimization of FISH (Fluorescence In-Situ Hybridization) and HER-2 DNA Detection by FISH in Breast cancer
EMPLOYMENT
Name of Organization Post held with
Pay Scale Job Profile Period
Shoukat Khanum Memorial Cancer Hospital & Research Center Lahore Research Associate level III Research Projects related to HPV, Ovarian and Breast Cancer
Guidance and training of MSc, M.Phil Students in Research 29-03-2010 to
28-03-2011
University of Health Sciences Lahore Assistant Professor To deliver Lectures and to Students
Supervision of MSc, M.Phil Students in Research Projects
To Conceive and Start new Research Projects related to Molecular Virology 01-04-2011 to
to date
SEMINARS & WORK SHOPS
2nd -3rd November 2010: 50th St. Andrew's Day Symposium (teleconference) on December 2-3, 2010 at Shaukat Khanum Memorial Cancer Hospital & Research Centre
26th -28th November 2010: 9th Symposium (Global Problem and local Solution) of Shaukat Khanum Memorial Cancer Hospital & Research Centre at Pearl continental hotel Lahore Pakistan
25th -28th March 2009: Symposium on ?Future Trends of molecular biological research in agriculture and health?
Organized by National Centre of Excellence in Molecular Biology (NCEMB), University of the Punjab, Lahore Pakistan
24th -28th March 2008: National Bioforum 2008
Organized by National Centre of Excellence in Molecular Biology (NCEMB), University of the Punjab, Lahore Pakistan
14th -19th Nov 2005: Pre 18th FAOBMB Symposium Satellite Workshop on Bioinformatics; organised and attended the workshop which includes lectures at (NCEMB), University of the Punjab, Lahore Pakistan with the international collaboration of FAOBMB, ISCB,
12th -24th January 2004 EU Asia Link Project Short course on Ecology, Sustainable Land Use jointly organized by university of Agriculture Faisalabad and Humboldt Universities Zu Berlin Germany
CURRENT RESEARCH INTERESTS
? Bimolecular interactions of different pathogen and host genes, their Protein structure and function involved in signalling transduction of different pathogenic pathways
? To study cells signalling pathways/Biomarkers involved in different cancerous diseases including Apoptosis, Cell proliferation, Oncogenesis, Angiogenesis etc.
? To study mechanism of new drugs/molecular medicine against different infectious and cancerous diseases
? Gene expression analysis and Comparative genomics in for Identification of Diagnostic markers and therapeutic targets for different diseases.
AREAS OF EXPERTISE
1. Molecular/Recombinant DNA Techniques:
Polymerase chain reaction (PCR), Real Time PCR, Sequencing, Multiplex PCR, Nested PCR, ISH In-situ Hybridization, FISH (Fluorescence In-situ Hybridization). IHC Chromatography, Gel electrophoresis (SDS-PAGE & Agarose), Cloning Techniques, Transformation, Microaaray technique, labeling of RNA with Cy3/Cy5, etc
2. Biochemical:
RNA extraction, Genomic DNA extraction, ELISA, Protein purification, RFLP, Western blotting, Southern blotting etc
3. Cell Culture Techniques:
Cell Culture (adherent and suspension) Mammalian cell culturing, transfection of cells with plasmids and siRNA, stable cell line generation, cells proliferation Assay, Disinfections and sterilization, Lab safety, , Biochemical examination,
4.
What I think of the idea behind WebmedCentral:
my intrest in rapid biomedical publication and shre of knowledge