I am Pharmacyst with MSc in Genetics and PhD in Molecular and Cellular Biology. Nowadays, I am a postdoctoral researcher on Centro de Excelencia en Metabolomoica y Bioanalisis in Madrid. I am engaged on the generation and evaluation of Leishmania sp. mutants using molecular (specially Proteomics and Metabolomics) and cellular approaches to understand gene function, control of gene expression and mechanisms of drug resistance.
In my PhD thesis I studied the effects of the overexpression of SL RNA (or spliced-leader), on the protozoan parasite Leishmania. This gene plays a central role in mRNA maturation in Trypanosomatid family and we noted that its overexpression induce the virulence decrease in the parasitic protozoa Leishmania. During my PhD, to understand the molecular mechanisms underlying the attenuation phenomenon, I had the opportunity to work with several methodologies applied to cellular and molecular biology of the parasite, such as pulsed field gel electrophoresis, western-blot, northern-blot, Southern-blot, 5 'and 3' RACE (Rapid amplification cDNA ends), primer extension, Real-time PCR, two-dimensional electrophoresis and proteomics, immunohistochemistry, FISH (Fluorescence in situ hibridization), scanning and transmission electron microscopy, fluorescence and confocal microscopy.
In my post-doc, to explore molecular mechanisms triggered by a ncRNA overexpression (called ODD3, unpublished) in Leishmania major, I have used Proteomic (2D electrophoresis and MALD-TOF mass spectrometry) and Metabolomic (GC/MS, HPLC/MS and CE/MS) approaches. In parallel to this project I'm investigating a Tunicamycin resistent L. braziliensis mutant generated by stepwise. Tunicamycin inhibits the N-glycosylation causing ER stress. To evaluate host-parasite interaction we are analysing cytokine/chemokine cascades (ELISA and Real Time) elicited by this mutant and the infectivity pattern in vivo and in vitro of this mutant compared with parental line. In order to discover ER stress signals induced by Tunicamicyn we are searching for alterations in genome (Pulsed-Field) and proteome (2D electrophoresis).
Assessor for Leishmania issues at DNApta
Postdoc at Centro de Excelencia en Metabolómica y Bioanálisis CEMBIO
Post doctoral at Universidade de São Paulo- FMRP- Depto. BioCel-Molecular Parasitology lab.
Post doc at Universidade de Sao Paulo- FMRP- Depto. BioCel-Molecular Parasitology lab.
PhD at Universidade de São Paulo
MSc at Universidade de Sao Paulo- FMRP- Dept. Genetics
Laboratory technician at Universidade de Sao Paulo- FMRP- Dept.Genetics.
Analysis of gene expression control
Drug discovery for neglected diseases
Molecular and Cell Biology
What I think of the idea behind WebmedCentral:
I think that it is a great idea. Nowadays, the biomedical publishing really needs to evolve and the WebmedCentral is given what the scientists needs " making the process of publishing more inclusive, efficient, accountable, economical, unbiased and transparent". The opportunity of publish without peer pressure and the proposal of break the barriers to publishing will reduce the timing of publication and give for every scientist an opportunity to contribute. The new way of publication is perfect fitted to the scientific age that we are living.