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Submited on: 29 Nov 2011 04:31:52 PM GMT
Published on: 30 Nov 2011 09:41:09 AM GMT
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Other Comments: 1. In the Methods section, should be mentioned albeit briefly, the detection and genotyping of virulence genes by PCR, and present the corresponding primer pairs.2. Some words are not separated in the text.3. Need to add references to 2009-11.
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Competing interests:
No
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Invited by the author to review this article? :
No -
Have you previously published on this or a similar topic?:
No
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References:
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Experience and credentials in the specific area of science:
I have no experience with Helicobacter, but I have with Campylobacter for 2 years, which are similar genders. - How to cite: Garcia G .Clinical Relevance of vacA, cagA, and iceA Genotypes of Helicobacter pylori[Review of the article 'Clinical Relevance of vacA, cagA, and iceA Genotypes of Helicobacter pylori ' by Ibrahim M].WebmedCentral 2012;2(12):WMCRW001205
Thank you for your professional review of this article. I'd like to respond to the some of the comments below:
Sub-topics are not appropriately placed: This is the website team work, some format has been changed when converted to PDF file and the website formate to columns.
At some points of the results section the authors simply refer to the tables without further explanation, these points should be explained on the text, for example "The presence of more than one genotype and their association with the clinical outcome is presented in table 3"
Response: more details are in the paragraph before on pages 3 and 4 under the title “The association between the vacA, cagA, and iceA2 genotypes with the clinical outcome”.
The authors mention that iceA2 was not correlated with duodenitis, which was present in 75% of the samples. Is this affirmation correct?
Response: The presence of iceA2 gene alone was only found in 2/9 cases, the other 7 cases were combined genotypes as presented in Table 3.
Responded by Prof. Laila Nimri on 13 Aug 2012 02:28:40 PM
Responded by Prof. Laila Nimri on 13 Aug 2012 02:28:40 PM
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The authors use PCR analysis to perform surveillance on the prevalence of a set of virulence genes in Helicobacter pylori strains collected from patients with gastritis from a hospital in Jordan. The paper correlates the presence/absence of these genes with clinical symptoms based on histological analysis of biopsy specimens. The paper is extremely important to determine the virulence profile of the strains circulating in that area/hospital.Several authors have documented the relationship between virulence genes and clinical presentation in H. pylori. Nevertheless, the claims are novel in the geographical context of the study. Information about prevalent strains in that area and the relationship between the genetic profile and disease outcome are important to aid medical personnel determine the course of treatment.
The authors cite relevant literature on the subject and the claims are in agreement with previous studies published on this subject. The methodology is correctly applied and appropriate to support the claims.
The paper would greatly benefit from a grammatical revision. Attention should be specially placed on typing errors, such as spacing between words. The "Results" section should be revised thoroughly. Sub-topics are not appropriately placed.
At some points of the results section the authors simply refer to the tables without further explanation, these points should be explained on the text, for example "The presence of more than one genotype asnd their association with the clinical outcome is presented in table 3"
The authors mention that iceA2 was not correlated with duodenitis, which was present in 75% of the samples. Is this affirmation correct?
No
No
No
I have been working with Gram negative pathogens for 10 years, mostly studying virulence factors and the interaction of the bacteria with the host.